Molecular profiling : methods and protocols /
edited by Virginia Espina and Lance A. Liotta.
- New York, N.Y. : Humana, c2012.
- xiii, 447 p. : ill. (some col.) ; 27 cm.
- Methods in molecular biology, 823 1064-3745 ; Springer protocols .
- Methods in molecular biology (Clifton, N.J.) ; v. 823. Springer protocols. .
Includes bibliographical references and index.
Tumor staging and grading: A primer -- Clinical trial design in the age of molecular profiling -- Personalized medicine: ethics for clinical trials -- Reduction of preanalytical variability in specimen procurement for molecular profiling -- The human side of cancer biobanking -- Introduction to genomics -- Genomic profiling: cDNA arrays and oligoarrays -- Genome-wide methylation profiling in archival formalin-fixed paraffin-embedded tissue samples -- An overview of microRNA methods: expression profiling and target identification -- Antibody validation by western blotting -- Laser capture microdissection: Arcturus(XT) infrared capture and UV cutting methods -- Antibody microarrays: Analysis of cystic fibrosis -- Tissue microarrays as a tool in the discovery and validation of predictive biomarkers -- Reverse-phase protein microarrays -- Serum low-molecular-weight protein fractionation for biomarker discovery -- Mass spectrometry-based biomarker discovery -- Mitochondrial proteome: toward the detection and profiling of disease associated alterations -- Adult neural stem cells: Isolation and propagation -- Evanescent-wave field imaging: An introduction to total internal reflection fluorescence microscopy -- Construction and hyperspectral imaging of quantum dot lysate arrays -- Microarray data analysis: Comparing two population means -- Bioinformatics/biostatistics: microarray analysis -- Structure-based functional design of drugs: From target to lead compound -- Personalized medicine: Changing the paradigm of drug development -- Grant writing tips for translational research -- Inventions and patents: a practical tutorial -- Regulatory approval pathways for molecular diagnostic technology -- Small business development for molecular diagnostics.